53BP1, Phospho (S25) Antibody

• Catalog Number: orb1529848
• Category: Antibodies
• Description: 53BP1, Phospho (S25) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: surrounding serine 25
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 1 - 4 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: 53BP1, Phospho (S25)
• UniProt ID: Q12888
• NCBI: NP_005648.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: p53-binding protein 1; tumor suppressor p53-bindin
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of Phospho 53BP1 (S25) by western blot.Samples: HEK293T cells that were mock treated or treated with NCS. Antibodies: Affinity purified rabbit anti-Phospho 53BP1 antibody orb1529848 at 0.04 µg/ml (A) and 0.1 µg/ml (B) for WB.

Detection of human and mouse Phospho 53BP1 (S25) by immunohistochemistry. Sample: FFPE sections of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Phospho 53bp1 (S25) used at a dilution of 1:1, 000.