5 Lipoxygenase/ALOX5 Antibody

• Catalog Number: orb234258
• Category: Antibodies
• Description: 5 Lipoxygenase/ALOX5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ALOX5 recombinant protein (Position: A120-R483). Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 77983 MW
• UniProt ID: P09917
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Arachidonate 5-lipoxygenase;5-LO;5-lipoxygenase;1.
• Note: For research use only
• Application notes: WB: The detection limit for ALOX5 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SGC Cell using Anti-ALOX5 Picoband antibody.

IHC(P) analysis of Mouse Brain Tissue using Anti-ALOX5 Picoband antibody.

IHC(P) analysis of Rat Brain Tissue using Anti-ALOX5 Picoband antibody.

IHC(P) analysis of Human Intestinal Cancer Tissue using Anti-ALOX5 Picoband antibody.

IHC(P) analysis of Human Mammary Cancer Tissue using Anti-ALOX5 Picoband antibody.