5-HT5A antibody

• Catalog Number: orb1474718
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 5-HT5A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT5A. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HTR5A
• Entrez: 25689, 15563, 3361
• UniProt ID: P47898, P35364, P30966
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: 5-hydroxytryptamine receptor 5A; 5-HT-5; 5-HT-5A;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 5-HT5A expression in mouse liver (A), mouse kidney (B), rat liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 55 kD)

Immunofluorescent analysis of 5-HT5A staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.