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Catalog Number | orb665810 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to 5-HT4 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT4. The exact sequence is proprietary. |
Dilution range | WB: 1-500-1000, IF: 1-100-500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | HTR4 |
Entrez | 3360, 15562, 25324 |
UniProt ID | Q62758, Q13639, P97288 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Anti-5-hydroxytryptamine receptor 4 antibody, anti Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of 5-HT4 expression in U87MG (A), rat heart (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 42 kD)
Immunofluorescent analysis of 5-HT4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FC, IF, IHC-P, WB | |
Bovine, Gallus | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Porcine | |
Rabbit | |
Polyclonal | |
Unconjugated |
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