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5-FAM-Azide
Catalog Number: orb532393
| Catalog Number | orb532393 |
|---|---|
| Category | Small Molecules |
| Description | Abs/Em = 494/520 nm |
| Form/Appearance | crystals; Color: light yellow to green |
| Purity | ≥ 90% (HPLC) |
| MW | Theoretical MW: 458.43 g/mol; Detected MW: 458.12 g/mol |
| Application notes | Spectroscopic Propertie: λabs 494 nm, λem 520 nm, ε 75.0 L mmol-1 cm-1. |
| Solubility (25°C) | DMF, DMSO, alcohols |
| Formula | C24H20N4O6 |
| SMILES | [N-]=[N+]=NCCCNC(=O)c1cc(c(cc1)c1c2ccc(=O)cc2oc2c1ccc(O)c2)C(=O)O |
| Storage | store at -20 °C |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
5-FAM-Azide [orb1734930]
≥ 90% (HPLC)
Theoretical MW: 458.43 g/mol; Detected MW: 458.12 g/mol
C24H20N4O6
5 mg5-FAM-Azide [orb1734931]
≥ 90% (HPLC)
Theoretical MW: 458.43 g/mol; Detected MW: 458.12 g/mol
C24H20N4O6
5 x 5 mg
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
