5-Ethynyl-UTP (5-EUTP)

SKU: orb532424

Description

5-Ethynyl-UTP (5-EUTP)

Images & Validation

• Application Notes:
  Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription. The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin); to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes); to crosslink the RNA to azide-functionalized biomolecules e.g.proteins. ; Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 508.16 g/mol (free acid); Detected MW: 507.97 g/mol (free acid)
• Purity: 95% (HPLC)
• Formula: C₁₁H₁₅N₂O₁₅P₃
• SMILES: n1([C@@H]2O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@H]([C@H]2O)O)c(=O)[nH]c(=O)c(c1)C#C
• Target: Spectroscopic Properties: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Storage & Handling

• Storage: Store at -20 C. Short-term exposure (up to 1 week cumulative) to ambient temperature possible.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Spectroscopic Properties: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)