5-Ethynyl-UTP (5-EUTP)

• Catalog Number: orb532424
• Category: Tools
• Description: 5-Ethynyl-UTP (5-EUTP)
• Concentration: 100 mM-110 mM
• Form/Appearance: solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 508.16 g/mol (free acid); Detected MW: 507.97 g/mol (free acid)
• SMILES: n1([C@@H]2O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@H]([C@H]2O)O)c(=O)[nH]c(=O)c(c1)C#C
• Formula: C₁₁H₁₅N₂O₁₅P₃
• Target: Spectroscopic Properties: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
• Storage: Store at -20 °C. Short-term exposure (up to 1 week cumulative) to ambient temperature possible.
• Note: For research use only
• Application notes: Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription. The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin); to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes); to crosslink the RNA to azide-functionalized biomolecules e.g.proteins. ; Spectroscopic Propertie: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Expiration Date: 12 months from date of receipt.