5-Ethynyl-uridine (5-EU)

SKU: orb65697

Description

Ethynyl-labeled uridine (5-EU) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging. Long term storage at < -20C. If stored as recommended, Biorbyt guarantees optimal performance of this product for 12 months after date of delivery.

Images & Validation

• Application Notes:
  RNA synthesis monitoring. ; Spectroscopic Propertie: max 286 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• CAS Number: 69075-42-9
• MW: Theoretical MW: 268.22 g/mol; Detected MW: 268.07 g/mol
• Purity: 99% (HPLC)
• Formula: C₁₁H₁₂N₂O₆
• SMILES: n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O

Storage & Handling

• Storage: store at -20 C
• Disclaimer: For research use only

Chemical structure of using 5-Ethynyl-uridine