5-Ethynyl-uridine (5-EU)

• Catalog Number: orb65697
• Category: Tools
• Description: Ethynyl-labeled uridine (5-EU) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging. Long term storage at < -20°C. If stored as recommended, Biorbyt guarantees optimal performance of this product for 12 months after date of delivery.
• CAS Number: 69075-42-9
• Form/Appearance: solid; Color: white to off-white
• Purity: ≥ 99% (HPLC)
• MW: Theoretical MW: 268.22 g/mol; Detected MW: 268.07 g/mol
• SMILES: n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O
• Formula: C₁₁H₁₂N₂O₆
• Solubility (25°C): Up to 200 mM in 1x PBS or water by heating the obtained, suspension for 1 minute by 70 °C, less soluble in DMSO
• Storage: store at -20 °C
• Note: For research use only
• Application notes: RNA synthesis monitoring. < br>< b>Spectroscopic Propertie: λmax 286 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Expiration Date: 12 months from date of receipt.

Chemical structure of using 5-Ethynyl-uridine