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5-Ethynyl-cytidine (5-EC)
Catalog Number: orb532336
| Catalog Number | orb532336 |
|---|---|
| Category | Small Molecules |
| Description | Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging. |
| Form/Appearance | solid; Color: white to off-white |
| Purity | ≥ 99% (HPLC) |
| MW | Theoretical MW: 267.24 g/mol; Detected MW: 267.09 g/mol |
| Application notes | RNA synthesis monitoring. Spectroscopic Propertie: λmax 294 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| Solubility (25°C) | DMSO |
| CAS Number | 65223-78-1 |
| Formula | C11H13N3O5 |
| SMILES | n1(cc(c(nc1=O)N)C#C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O |
| Storage | store at -20 °C |
| Hazard Information | Non-Toxic |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
