5-Ethynyl-cytidine (5-EC)

• Catalog Number: orb532336
• Category: Small Molecules
• Description: Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.
• Form/Appearance: solid; Color: white to off-white
• Purity: ≥ 99% (HPLC)
• MW: Theoretical MW: 267.24 g/mol; Detected MW: 267.09 g/mol
• Application notes: RNA synthesis monitoring. < br>< b>Spectroscopic Propertie: λmax 294 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Solubility (25°C): DMSO
• CAS Number: 65223-78-1
• Formula: C₁₁H₁₃N₃O₅
• SMILES: n1(cc(c(nc1=O)N)C#C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O
• Storage: store at -20 °C
• Hazard Information: Non-Toxic
• Note: For research use only