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Catalog Number | orb532331 |
---|---|
Category | Tools |
Description | 5-DBCO-dU (5-Dibenzylcyclooctyne-2'-deoxyuridine) can potentially be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis in proliferating cells. 5-DBCO-dU is cell permeable and may incorporate into replicating DNA instead of its natural analog thymidine. The resulting DBCO-functionalized DNA can subsequently be detected via Cu(I)-free Click Chemistry that offers the choice to: introduce a Biotin group for subsequent purification tasks (via Azides of Biotin); introduce a fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes). |
Form/Appearance | solid; Color: colorless to slightly yellow |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 568.58 g/mol (free acid); Detected MW: 568.20 g/mol (free acid) |
SMILES | c1(=O)[nH]c(=O)n(cc1C#CCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)CO |
Formula | C31H28N4O7 |
Storage | store at -20 °C |
Note | For research use only |
Application notes | DNA synthesis monitoring (potentially).
Spectroscopic Propertie: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
Expiration Date | 12 months from date of receipt. |
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