5-Azido-C3-UTP

SKU: orb178349

Description

5-Azido-C3-UTP

Images & Validation

• Application Notes:
  Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested). For a comprehensive protocol please refer to PP-501 or PP-501-Cy3/Cy5. 3'-Azide-Labeling of T7 promotor-containing oligonucleotides. The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively); to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively); to crosslink the RNA to azide-or alkyne functionalized biomolecules e.g.proteins. ; Spectroscopic Propertie: λmax 260 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 567.23 g/mol (free acid); Detected MW: 567.02 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₁₂H₂₀N₅O₁₅P₃
• SMILES: C(CCc1cn(c(=O)[nH]c1=O)[C@H]1[C@@H]([C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N=[N+]=[N-]

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only