4EBP1 Antibody

• Catalog Number: orb1530099
• Category: Antibodies
• Description: 4EBP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: between 75 and C-term
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:6,000; IHC - 1:5,000 - 1:20,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: 4EBP1
• UniProt ID: Q13541
• NCBI: NP_004086.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: 4EBP1; 4E-BP1; BP-1; eIF4E-binding protein 1; euka
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of 4EBP1 by western blot.Samples: Whole cell lysate from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody: Affinity purified rabbit anti-4EBP1 (orb1530099) used at 0.25 µg/ml.

Detection of human 4EBP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20, 000 (0.05µg/ml).