4EBP1 antibody

• Catalog Number: orb1474544
• Category: Antibodies
• Description: Rabbit monoclonal antibody to 4EBP1
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IH, IP, WB
• Reactivity: Hamster, Human, Mouse, Rat
• Immunogen: A synthetic peptide of human eIF4EBP1
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: EIF4EBP1
• Entrez: 116636, 1978, 13685
• UniProt ID: Q13541, Q62622, Q60876
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: Eukaryotic translation initiation factor 4E-bindin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 4EBP1 expression in mouse heart (A), Jurkat (B), C6 (C), CHOK1 (D) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 15-20 kD)

Immunohistochemical analysis of 4EBP1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.9). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.