4E-BP1 (phospho-Thr69) antibody

• Catalog Number: orb767260
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 4E-BP1 (phospho-Thr69)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69. AA range:35-84
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 12
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-EIF4EBP1 antibody, anti-Eukaryotic translatio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 4E-BP1 (Phospho-Thr69) Antibody.

Immunohistochemistry analysis of paraffin-embedded human brain carcinoma, using 4E-BP1 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using 4E-BP1 (Phospho-Thr69) Antibody. The lane on the right is blocked with the phospho peptide.