41886 Antibody

• Catalog Number: orb1249029
• Category: Antibodies
• Description: 41886 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-RNKLTRESGTD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in Human and Mouse Heart lysates (calculated MW of 55.1kDa according to NP_004565.1 and 53.0kDa according to NP_536341). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: 41886
• UniProt ID: O43236
• NCBI: NP_536341.1, NP_004565.1, NP_001185642.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SEPT4, septin 4, ARTS, BRADEION, CE5B3, H5, MART,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in Human and Mouse Heart lysates (calculated MW of 55.1kDa according to NP_004565.1 and 53.0kDa according to NP_536341). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249029 (1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249029 (3.75 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.