You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1239073 |
---|---|
Category | Antibodies |
Description | 3CL-PRO Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Virus |
Isotype | IgG |
Immunogen | Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (orb1239073) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein. |
Concentration | 1 mg/mL |
Dilution range | IHC: 0.5 μg/mL; WB: 0.5 μg/mLAntibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP5 recombinant protein by ELISA and WB. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | 3CL-PRO |
UniProt ID | P0DTC1 |
NCBI | YP_009742612.1 |
Storage | SARS-CoV-2°COVID-19) 3CL-PRO (NSP5) antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | 3C-like proteinase, 3CLp, nsp5, Non-structure prot Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) 3L-PRO in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) 3L-PRO (NSP5) antibody (orb1239073, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 3L-PRO (NSP5) protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein. Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, orb1239073. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein (orb1231584) as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (orb1231584). Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), orb1239073, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL and. Lane 2: 1 µg/mL.
ELISA | |
Virus | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating