38961 Antibody

• Catalog Number: orb1249616
• Category: Antibodies
• Description: 38961 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DEVNAFKQRKTA
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 50kDa band observed in Human Testis, Thyroid Gland and MOLT4 cell lysates (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.03-1ug/ml.Immunohistochemistry:In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: 38961
• UniProt ID: Q14141
• NCBI: NP_665798.1, NP_665801.1, NP_055944.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SEPT6, septin 6, KIAA0128, MGC16619, MGC20339, SEP
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 50kDa band observed in Human Testis, Thyroid Gland and MOLT4 cell lysates (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.03-1ug/ml.Immunohistochemistry:In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249616 (1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249616 (3.8 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.