37500 Antibody

• Catalog Number: orb1246629
• Category: Antibodies
• Description: 37500 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: SKQQPTQFINPET-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 40kDa band observed in Human Testis lysates (calculated MW of 41.5kDa according to NP_001008491.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast, Tonsil and Brain (Cortex). Recommended concentration: 2.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: 37500
• UniProt ID: Q15019
• NCBI: NP_001008491.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SEPT2, septin 2, DIFF6, KIAA0158, NEDD5, Pnutl3, h
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 40kDa band observed in Human Testis lysates (calculated MW of 41.5kDa according to NP_001008491.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast, Tonsil and Brain (Cortex). Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246629 (0.05 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246629 (2.5 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246629 (2.5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246629 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.