2-Ethynyl-adenosine

• Catalog Number: orb154335
• Category: Tools
• Description: 2-Ethynyl-labeled adenosine (2-EA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. 2-EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I, II and III and posttranscription-ally by poly(A) polymerase instead of their natural analolg adenosine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.
• Form/Appearance: solid; Color: white to off-white
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 291.26 g/mol; Detected MW: 291.10 g/mol
• SMILES: c12c(nc(nc2n(cn1)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O)C#C)N
• Formula: C₁₂H₁₃N₅O₄
• Solubility (25°C): up to 4.0 mM in 1x PBS
• Storage: store at -20 °C
• Note: For research use only
• Application notes: mRNA poly(A) tail synthesis monitoring.
• Expiration Date: 12 months from date of receipt.

Chemical structure of using 2-Ethynyl-Adenosine (Small Molecules)