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Catalog Number | orb196244 |
---|---|
Category | Antibodies |
Description | 15 Lipoxygenase 1/ALOX15 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 74804 MW |
UniProt ID | P16050 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Arachidonate 15-lipoxygenase;15-LOX;15-LOX-1;1.13. Read more... |
Note | For research use only |
Application notes | WB: The detection limit for ALOX15 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of ALOX15 using anti-ALOX15 antibody.Lane 1:rat lung tissue; 2:rat thymus tissue; 3:rat spleen tissue; 4:mouse lung tissue; 5:mouse testis tissue; 6:mouse thymus tissue.
IHC analysis of ALOX15 using anti-ALOX15 antibody. ALOX15 was detected in a paraffin-embedded section of mouse lung tissue.
IHC analysis of ALOX15 using anti-ALOX15 antibody. ALOX15 was detected in a paraffin-embedded section of rat lung tissue.
IHC analysis of ALOX15 using anti-ALOX15 antibody. ALOX15 was detected in a paraffin-embedded section of human intestine cancer tissue.
IHC analysis of ALOX15 using anti-ALOX15 antibody. ALOX15 was detected in a paraffin-embedded section of human mammary cancer tissue.
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