14-3-3/YWHAZ Mouse Monoclonal Antibody

• Catalog Number: orb500615
• Category: Antibodies
• Description: 14-3-3/YWHAZ Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1B6
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b
• Immunogen: Recombinant human YWHAZ protein
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: YWHAZ
• UniProt ID: P63104
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (YWHAZ) Polyclonal Antibody, Unconjugated (orb500615) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 40 ug, A549 (Human) Cell Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Panc-1 (Human) Lysate at 40 ug, Primary: Anti-YWHAZ at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kD, Observed band size: 23 kD.