Human IL-2 Protein

• Catalog Number: orb1087555
• Category: Proteins
• Description: Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
• Reactivity: Human
• Tag: N-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 41.9 kDa
• Target: IL-2
• Source: Human IL-2 Protein, Fc Tag (orb1087555) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-2 R alpha, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-2 Protein, Fc Tag with a linear range of 0.1-2 ng/mL (QC tested).
• Expression Region: Ala 21 - Thr 153
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000577.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL2,TCGF,lymphokine,Interleukin 2
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 41.9 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-2 R alpha, His Tag (Cat. No. orb257613) at 5 μg/mL (100 μL/well) can bind Human IL-2 Protein, Fc Tag (Cat. No. orb1087555) with a linear range of 0.1-2 ng/mL (QC tested).