Human IFN-alpha 1 Protein

• Catalog Number: orb735041
• Category: Proteins
• Description: Interferon alpha-1 is also known as IFN-alpha-1/13, Interferon alpha-D, LeIF D and IFNA1, belongs to the alpha / beta interferon family. Interferons alpha-1 and alpha-13 have identical protein sequences. Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha can either suppress or promote the development of autoimmune diabetes. It is likely that IFN-alpha plays a complex role in the etiology of type 1 diabetes.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 21.3 kDa
• Target: IFN-alpha 1
• Source: Human IFN-alpha 1, His Tag (orb735041) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1).
• Expression System: HEK293
• Biological Activity: Loaded Human IFN-alpha 1, His Tag on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: Cys 24 - Glu 189
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.3 kDa. The protein migrates as 22-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Loaded Human IFN-alpha 1, His Tag (Cat. No. orb735041) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. orb257558) with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).