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| Catalog Number | orb98335 |
|---|---|
| Category | Antibodies |
| Description | Rat monoclonal to Heparan Sulphate Proteoglycan |
| Species/Host | Rat |
| Clonality | Monoclonal |
| Clone Number | A7L6 |
| Tested applications | ICC, IHC-Fr, IHC-P, IP, WB |
| Reactivity | Bovine, Fish, Human, Mouse, Rat |
| Isotype | IgG2a |
| Form/Appearance | The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide. |
| Conjugation | Unconjugated |
| Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. |
| UniProt ID | P98160 |
| Source | A7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG. |
| Storage | Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product |
| Buffer/Preservatives | The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of mouse with Hurler disease using Heparan Sulphate Proteoglycan antibody.
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Heparan Sulphate Proteoglycan antibody (orb98335)
We stained fresh frozen retinal/eye tissues. We tested both 1:100 and 1:200, and both worked - 1:100 was a bit brighter qualitatively, but we did not measure it. We tested both no fix and 4% para. Both work fine, although 1:200 with 4% para fix was noticeably dimmer.
- Linda McLoon/University of Minnesota
Applications
IHC
Rating
- 5 stars