Versican Antibody

SKU: orb99108

Description

Mouse Anti-Mouse Versican Monoclonal IgG1

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-439 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N351/23 (Formerly sold as S351-23)
• Immunogen: Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
• Target: Versican
• Molecular Weight: 350kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Versican, CSPG2, VCAN, PG-M, PGM, PG-M proteoglycan, Chondroitin sulfate proteoglycan 2, Chondroitin sulfate proteoglycan core protein 2, Large aggregating proteoglycan, Large fibroblast proteoglycan, Glial hyaluronate-binding protein, Glial hyaluronate binding protein, GHAP, ERVR, Versican core protein, Versican proteoglycan, Versican V0, Versican V1, Versican V2, Versican V3, Versican V4, V1 Neo, WGN, WGN

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone N351/23. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Versican Antibody (D) Composite.

Quick Database Links

Gene Symbol

Versican

UniProt

Q62059

NCBI Gene ID

13003

RefSeq:AAH96495