Thymidine Phosphorylase Antibody / PD-ECGF

SKU: orb386150

Description

Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Images & Validation

• Tested Applications: IHC-P, IP, WB
• Dilution range: Western blot: 0.5-1ug/ml,Immunoprecipitation: 0.5-1ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Mouse, Rat
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidine Phosphorylase antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: P-GF.44C
• Immunogen: Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human spleen lysate with Thymidine Phosphorylase antibody (clone P-GF.44C). Predicted molecular weight ~55 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Thymidine Phosphorylase Antibody (clone P-GF.44C). Confirmation of Integrity and Purity of the Antibody.

IHC testing of FFPE human placenta with Thymidine Phosphorylase antibody (clone P-GF.44C). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

P19971