SOX2 Antibody

SKU: orb1252216

Description

SOX2 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Reactivity: Human
• Application Notes:
  ELISA: 2-4 µg/mL; order BSA-free formulation for coatingFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLWestern blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RTOptimal dilution of the SOX2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: SOX2/1792
• Immunogen: Amino acids 176-305 of the human protein were used as the immunogen for the SOX2 antibody.
• Target: SOX2
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2

IHC testing of FFPE human cervical carcinoma with SOX2 antibody (clone SOX2/1792). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human lung carcinoma with SOX2 antibody (clone SOX2/1792). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of 1) partial human protein and 2) human NCCIT lysate with SOX2 antibody (clone SOX2/1792). Predicted molecular weight ~34 kDa.

Quick Database Links

Gene Symbol

SOX2

UniProt

P48431