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Description
Research Area
Epigenetics & Chromatin, Stem Cell & Developmental Biology
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/50 ChIP: 1/20 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | BT20X8M7 |
| Immunogen | A synthesized peptide derived from human Oct4 |
| Target | POU5F1 |
| Molecular Weight | Calculated MW: 39 kDa; Observed MW: 45 kDa |
| Purification | Affinity Chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% rAlbumin |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF 3; OTF 4; OTF-3; OTF3; OTF4; PO5F1_HUMAN; POU class 5 homeobox 1; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain; class 5; transcription factor 1; POU5F1.
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Quick Database Links
Gene Symbol
POU5F1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation

















