AVS Bio - mouse citrullinated fibrinogen antibody

SKU: MQ13.104

Description

Antibody against fibrinogen. Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deiminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:1000 – 1:2000. IB: 1:200 – 1:1000. IHC: 1:100 – 1:500. Other applications: since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
• Reactivity: Human

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: 23H2
• Immunogen: Deiminated murine fibrinogen peptide.
• Target: citrullinated fibrinogen
• Purification: Protein A purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: lyophilized
• Buffer/Preservatives: The antibody has been lyophilized in a 10 mM ammonium bicarbonate buffer.
• Expiration Date: For shelf life and expiration details, please refer to the Certificate of Analysis (CoA).
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

citrullinated fibrinogen