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Description
Research Area
Cell Biology, Epigenetics & Chromatin
Images & Validation
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Item 1 of 2
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | BHEKX31 |
| Immunogen | A synthesized peptide derived from human Phospho-SMC1 (S957) Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair . ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint. SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment. |
| Target | Structural maintenance of chromosomes protein 1A |
| Molecular Weight | 15 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | 0.5mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Red fluorescent protein eqFP611; GFP-like chromoprotein
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Phospho-SMC1 alpha (Ser957) Recombinant Rabbit Monoclonal Antibody [orb2561131]
ICC, IF, IHC-Fr, IHC-P
Human
Human, Mouse, Rat
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl, 25 μl
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Quick Database Links
Gene Symbol
Structural maintenance of chromosomes protein 1A
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
Available Sizes
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