Losartan potassium

SKU: orb1310826

Description

Losartan potassium is a selective, competitive antagonist of the angiotensin II type 1 (AT1) receptor. It is widely used in research to study the renin-angiotensin system in models of hypertension, cardiovascular disease, and renal function, both in vitro and in vivo.

Research Area

Metabolism Research

Key Properties

• CAS Number: 124750-99-8
• MW: 461.01
• Purity: 99.98%
• Formula: C₂₂H₂₂ClKN₆O
• SMILES: [K+].CCCCc1nc(Cl)c(CO)n1Cc1ccc(cc1)-c1ccccc1-c1nnn[n-]1
• Target: RAAS
• Solubility: H2O:46.1 mg/mL (100 mM);DMSO:250 mg/mL (542.29 mM)

Bioactivity

• Target IC50:
  AT1 receptor:20 nM
• In Vitro:
  Losartan competes with the binding of angiotensin II to AT1 receptors, with an IC50 of 20 nM. At 40 μM, losartan affects ISC and prevents the effect of ANGII on ISC. It significantly reduces Ang II-mediated cell proliferation in endometrial cancer cells, and in combination with anti-miR-155, it exhibits a significantly greater antiproliferative effect compared to each drug alone.
• Cell Research:
  An MTT assay is used to measure cell proliferation and viability. For the assay, 5000 cells in 200 μL media per well are seeded in a 96 well plate. After overnight incubation to allow for cell attachment, the medium is removed by suction. MTT at 1 mg/mL concentration in serum-free medium is added and then incubated for 4 h at 37°C. After removal of MTT solution, 100 μL of DMSO is added to dissolve formazan crystals. Absorbance at 570 nm and at 600 nm as a reference is then measured using a microplate reader. The difference in absorbance is thus relative to the extent of cell survival.

Storage & Handling

• Storage: store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MK954, MK-954, MK 954, Losartan, Losartan potassium, inhibit, Inhibitor, DuP-753, DuP 753, DuP-753 potassium, DuP753, AT1 receptor, Angiotensin Receptor

Compound Identifiers

PubChem CID

11751549