VSIG4 Rabbit Polyclonal Antibody

SKU: orb11565

Description

VSIG4 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human VSIG4 (81-160/399aa)
• Target: VSIG4
• Molecular Weight: 33 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

V-set and immunoglobulin domain-containing protein 4; CRIg; V set and immunoglobulin domain containing 4; Z39IG; VSIG4_HUMAN.

Sample: Lung (Mouse) Lysate at 30 ug, Placenta (Mouse) Lysate at 30 ug, Primary: Anti-VSIG4 (orb11565) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-VSIG4 (orb11565) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-VSIG4 (orb11565) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Tissue/Cell: Rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-VSIG4 Polyclonal Antibody, Unconjugated (orb11565) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

VSIG4

UniProt

Q9Y279

References

Yuan Liao, Chaonan Deng, Xichuan Wang VSIG4 ameliorates intestinal inflammation through inhibiting macrophages NLRP3 inflammasome and pyroptosis Tissue Cell, 86, 102285 (2024)

PubMed: 38113649

Applications

IHC

Reactivity

Human