Vimentin Rabbit Polyclonal Antibody

• Catalog Number: orb500800
• Category: Antibodies
• Description: Vimentin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Mouse, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human Vimentin (1-466/466aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: VIM
• UniProt ID: P08670
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vim
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with Vimentin polyclonal antibody, unconjugated (orb500800) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb500800) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb500800) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.