Vimentin Antibody

• Catalog Number: orb1151549
• Category: Antibodies
• Description: Mouse monoclonal antibody to Vimentin
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human Vimentin protein was used as the immunogen for the Vimentin antibody.
• UniProt ID: P08670
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Vimentin antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rVIM/6575
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human U-87 MG cell lysate using Vimentin antibody (clone rVIM/6575). Predicted molecular weight ~53 kDa.

IHC staining of FFPE human tonsil tissue with Vimentin antibody (clone rVIM/6575). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with Vimentin antibody (clone rVIM/6575). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone rVIM/6575) as confirmation of integrity and purity.