SARS-CoV Matrix Antibody

• Catalog Number: orb1239027
• Category: Antibodies
• Description: SARS-CoV Matrix Antibody
• Target: M
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: SARS-CoV-2/SARS-CoV Matrix Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: SARS-CoV-2/SARS-CoV Matrix Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-SARS-CoV-2/SARS-CoV Matrix antibody (orb1239027) was raised against a peptide corresponding to 15 amino acids near the carboxy-terminus of SARS-CoV Matrix protein. The immunogen is located within the last 50 amino acids of SARS-CoV Matrix.
• UniProt ID: O62114
• Tested applications: ELISA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Ma
• Research Area: Infectious Disease & Virology
• Note: For research use only
• NCBI: QHO62114.1

Western Blot Validation with SARS-CoV-2 Matrix Recombinant Protein. Loading: SARS-CoV-2 Matrix recombinant protein. Antibodies: SARS-CoV-2/SARS-CoV Matrix (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 10 ng Matrix protein, Lane 2: 25 ng Matrix protein and Lane 3: 50 ng Matrix protein.

ELISA Test. Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, orb1239027. A direct ELISA was performed using Matrix recombinant protein as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Detection range is from 8 ng/mL to 2000 ng/mL.

ELISA Test. Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, orb1239027 (1 µg/mL). A direct ELISA was performed using immunogen (orb1239027) as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.