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SAFB Antibody
Catalog Number: orb1259371
| Catalog Number | orb1259371 |
|---|---|
| Category | Antibodies |
| Description | SAFB Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human SAFB (NP_002958.2). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| Target | SAFB |
| UniProt ID | Q15424 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | SAFB, HAP, HET, Scaffold attachment factor B, SAB- Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SAFB antibody (orb1259371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.
Immunofluorescence analysis of U2OS cells using SAFB antibody (orb1259371) at dilution of 1:100. Blue: DAPI for nuclear staining.
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IF, IHC, IHC-P, IP, WB
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