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RNF40 Antibody
SKU: orb1257006
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ChIP, IF, IHC, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF40 (NP_055586.1). |
| Target | RNF40 |
| Molecular Weight | Observed: 140kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−RNF40, BRE1B, DKFZp686K191, KIAA0661, MGC13051, RBP95, STARING
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Western blot analysis of extracts of various cell lines, using RNF40 antibody (orb1257006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using RNF40 antibody (orb1257006).
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF40 antibody (orb1257006) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Quick Database Links
Gene Symbol
RNF40
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ChIP
Chromatin Immunoprecipitation
RNF40 Antibody (orb1257006)
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