Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast

• Catalog Number: orb1906113
• Category: Proteins
• Description: Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Purity: > 95 % by SDS-PAGE and 90% by SEC-HPLC analyses.
• Protein Sequence: MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
• MW: Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.
• Expression System: Yeast
• Endotoxins: Less than 0.1 EU/ug of rRtVEGF164, Yeast as determined by LAL method.
• Source: Yeast
• Biological Activity: Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/ml.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Note: For research use only
• Expiration Date: 6 months from date of receipt.