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| Catalog Number | orb2993140 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Angiotensin-Converting Enzyme 2 is produced by our Mammalian expression system and the target gene encoding Gln18-Ser740 is expressed with a human IgG1 Fc tag at the C-terminus. |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Form/Appearance | Supplied as a 0.2 μm filtered solution of 20mM Tris–HCl, 300mM NaCl, 10% Glycerol, 100mM Glycine, pH 7.4. |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) |
| UniProt ID | Q9BYF1 |
| MW | Predicted: 110.5 KDa. Observed: 110-140 KDa, reducing conditions |
| Expression System | Human Cells |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized 2019-nCoV S Protein RBD-SD1-mFc(Cat#DRA38) at 2μg/ml (100 μl/well) can bind Human ACE-2-FC(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 25-250 ng/ml. (QC verified) Immobilized 2019-nCoV S-trimer Protein-His(Cat#DRA107) at 2μg/ml (100 μl/well) can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 23.83 ng/ml. Immobilized 2019-nCoV S1 Protein-His(Cat#DRA133) at 5μg/ml (100 μl/well)can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 21.73 ng/ml. Immobilized 2019-nCoV S-trimer Protein -His(Cat#DRA136) at 2μg/ml (100 μl/well)can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 11.98 ng/ml. Immobilized 2019-nCoV S-trimer Protein-His(Cat#DRA153) at 5μg/ml (100 μl/well)can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 30.11 ng/ml. Immobilized 2019-nCoV S-trimer Protein-His(Cat#DRA154) at 5μg/ml (100 μl/well)can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 95.28 ng/ml. Immobilized 2019-nCoV S-trimer Protein-His(Cat#DRA157) at 5μg/ml (100 μl/well)can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 50.33 ng/ml. Immobilized 2019-nCoV S-trimer Protein-His(Cat#DRA168) at 2μg/ml (100 μl/well)can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 0.133 ug/ml. Immobilized SARS-CoV-2 S-trimer Protein-His(Cat#DRA175) at 2μg/ml (100 μl/well)can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 0.19 ng/ml. Immobilized SARS-CoV-2 S-trimer Protein-His(Cat#DRA177) at 5μg/ml (100 μl/well)can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 29.80 ng/ml. Immobilized 2019-nCoV S1 Protein(Cat#DRA35) at 2μg/ml (100 μl/well) can bind Human ACE-2-Fc(Cat#orb2993140). The ED50 of Human ACE-2-Fc(Cat#orb2993140) is 15-150 ng/ml. Immobilized SARS-CoV-2 S Protein RBD-SD1-His (Cat#DRA42) at 5μg/ml (100 μl/well) can bind Human ACE-2-Fc(Cat#orb2993140).The ED50 of Recombinant Human ACE-2-Fc(Cat#orb2993140) is 76 ng/ml. Immobilized 2019-nCoV S1-His (Cat#DRA47) at 5μg/ml (100 μl/well) can bind Human ACE-2- Fc(Cat#orb2993140).The ED50 of Recombinant Human ACE-2-Fc(Cat#orb2993140) is 58.3 ng/ml. |
| Expression Region | Gln18-Ser740 |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Alternative names | Angiotensin-Converting Enzyme 2; ACE-Related Carbo Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 109.7 kDa after removal of the signal peptide. | |
Mammalian |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 84.63 KDa. Observed: 103 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 110.6 KDa. Observed: 120-140 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 110 KDa. Observed: 110-140 KDa, reducing conditions |
Biotin | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 86.2 KDa. Observed: 110-140 KDa, reducing conditions |
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