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| Catalog Number | orb2993542 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Angiotensin-Converting Enzyme 2 is produced by our Mammalian expression system and the target gene encoding Gln18-Ser740 is expressed with a 6His tag at the C-terminus. |
| Conjugation | Unconjugated |
| Tag | C-6His |
| Form/Appearance | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 300mM NaCl, 1mM ZnCl2, 10% Glycerol, pH 7.4. |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| UniProt ID | Q9BYF1 |
| MW | Predicted: 84.63 KDa. Observed: 103 KDa, reducing conditions |
| Expression System | Human Cells |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Biological Origin | Human |
| Biological Activity | BLI: Loaded 2019-nCoV S Protein RBD-mFc(Cat#DRA32) on AMC Biosensor, can bind Human ACE-2-His(Cat#orb2993542) with an affinity constant of 2.06 nM as determined in BLI assay. |
| Expression Region | Gln18-Ser740 |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Alternative names | Angiotensin-Converting Enzyme 2; ACE-Related Carbo Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 109.7 kDa after removal of the signal peptide. | |
Mammalian |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 110.6 KDa. Observed: 120-140 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 110 KDa. Observed: 110-140 KDa, reducing conditions |
Biotin | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 86.2 KDa. Observed: 110-140 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) | |
Predicted: 110.5 KDa. Observed: 110-140 KDa, reducing conditions |
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