Rat Urocortin 3 (UCN3) ELISA Kit

• Catalog Number: orb781327
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Urocortin 3(UCN3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Urocortin 3(UCN3).
• Reactivity: Rat
• Concentration: 1000 pg/mL
• Tested applications: ELISA
• Application notes: standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat UCN3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat UCN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat UCN3 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 4.56 pg/mL
• Sample Types: Serum, plasma and other biological fluids
• Alternative names: SCP ELISA Kit, SPC ELISA Kit, UCNIII ELISA Kit, St
• Research Area: Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.