Rabbit anti-RBM22 Antibody

SKU: orb1523926

Description

RBM22 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 175 and 225
• Target: RBM22

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

Cwc2; zinc finger CCCH domain-containing protein 16; ZC3H16; RNA-binding motif protein 22; pre-mRNA-splicing factor RBM22; fSAP47; functional spliceosome-associated protein 47

Detection of mouse RBM22 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM22 antibody (orb1523926) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RBM22 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, OVCAR-8, and SR cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM22 antibody (orb1523926) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human RBM22 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM22 antibody (orb1523926) used for IP at 6 µg per reaction. RBM22 was also immunoprecipitated by a previous lot of this antibody (orb1523926) and a second antibody against a different epitope of RBM22.

Quick Database Links

Gene Symbol

RBM22

UniProt

Q9NW64

RefSeq (Protein):NP_060517.1