Rabbit anti-PolA1 Antibody

SKU: orb1525881

Description

PolA1 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1175 and 1225
• Target: PolA1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

POLA; polymerase (DNA-directed), alpha (70kD); polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA directed), alpha 1, catalytic subunit; NSX; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; p180

Detection of human PolA1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PolA1 antibody orb1525881 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Quick Database Links

Gene Symbol

PolA1

UniProt

P09884

RefSeq (Protein):NP_058633.2