Rabbit anti-FANCA Antibody

SKU: orb1527444

Description

FANCA Antibody

Images & Validation

• Tested Applications: FC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1405 and C-terminus
• Target: FANCA
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

Fanconi anemia, type 1; FA; Protein FACA; Fanconi anemia, complementation group H; Fanconi anemia group A protein; Fanconi anemia complementation group A; FA-H; FAH; FACA; FA1; FAA; FANCH

Detection of human FANCA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, A-549, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCA antibody (orb1527444) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human FANCA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FANCA antibody (orb1527444) used for IP at 6 µg per reaction.

Detection of human FANCA (shaded) in HDLM-2 cells by flow cytometry.Antibody: Rabbit anti-FANCA polyclonal antibody (orb1527444) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

FANCA

UniProt

O15360

RefSeq (Protein):NP_000126.2