Rabbit anti-CD3E Recombinant Monoclonal Antibody

SKU: orb1519962

Description

CD3E Antibody

Images & Validation

• Tested Applications: FC, IF, IHC, IP, WB
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BL-298-5D12
• Immunogen: Between 157 and C-terminus
• Target: CD3E
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Disclaimer: For research use only

Alternative Names

CD3-epsilon; TCRE; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; CD antigen CD3e; IMD18; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T3E

Detection of human CD3E (orange) , CD8 alpha (red) , and PD-L1 (green) in FFPE breast carcinoma by IHC-IF. Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (orb1519962) , rabbit anti-CD8 alpha recombinant monoclonal, rabbit anti-PD-L1 recombinant monoclonal. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

CD3E

UniProt

P07766

RefSeq (Protein):NP_000724.1