Rabbit anti-Bub1 Antibody

• Catalog Number: orb1530292
• Category: Antibodies
• Description: Bub1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Bub1
• UniProt ID: O43683
• NCBI: NP_004327.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hBUB1; BUB1 budding uninhibited by benzimidazoles
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Bub1 by western blot.Samples: Whole cell lysate from HeLa treated with nacodazole (+) or mock treated (-), HEK293T. and Jurkat cells. Antibody: Affinity purified rabbit anti-Bub1 antibody orb1530292 at 0.4 µg/ml.

Detection of human Bub1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Bub1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human Bub1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells treated with nocodazole. Antibodies: Affinity purified rabbit anti-Bub1 antibody.