Anti-BUB1 Antibody

• Catalog Number: orb412044
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BUB1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant full length protein of human BUB1
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BUB1
• Entrez: 12235, 699
• UniProt ID: O43683, O08901
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-BUB1L antibody, anti-Mitotic checkpoint serin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BUB1 expression in HepG2 (A), MCF7 (B), Hela (C), mouse testis (D) whole cell lysates. (Predicted band size: 115; 120; 122 kD; Observed band size: 133 kD)

Immunohistochemical analysis of BUB1 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.