PLIN3 Mouse Monoclonal Antibody

• Catalog Number: orb783340
• Category: Antibodies
• Description: PLIN3 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: D2A3
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: IgG2a,k
• Immunogen: KLH conjugated synthetic peptide derived from human PLIN3 (1-434/434aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1:20-50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: PLIN3
• UniProt ID: O60664
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TIP47; PLIN3_HUMAN; 47 kDa MPR binding protein; Ca
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HUVEC. Primary Antibody (green line): Mouse Anti-PLIN3 antibody (orb783340), dilution: 1:25, Isotype Control Antibody (blue line): Mouse IgG, Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1:200, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Hela cell lysates, Lane 2: K562 cell lysates, Lane 3: Daudi cell lysates, Primary: Anti-PLIN3 (orb783340) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.