PLIN3 Antibody

• Catalog Number: orb1925838
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1651CT157.85.94
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: IgG2a,k
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:2000
• Conjugation: Unconjugated
• MW: 47075 Da
• Target: This PLIN3 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-434 amino acids from human PLIN3.
• UniProt ID: O60664
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Perilipin-3, 47 kDa mannose 6-phosphate receptor-b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PLIN3 Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: Daudi whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling PLIN3 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. The nuclear counter stain is DAPI (blue).