PDPK1 Antibody

SKU: orb229750

Description

Mouse monoclonal antibody to PDPK1

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution range: WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 3H3D9
• Immunogen: Purified recombinant fragment of human PDPK1 (AA: 457-556 expressed in E. Coli.
• Target: PDPK1
• Molecular Weight: 63.2kDa

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

PDK1; PDPK2; PDPK2P; PRO0461

Immunofluorescence analysis of Hela cells using PDPK1 antibody

Western blot analysis of human recombinant protein using PDPK1 antibody

Western blot analysis of MCF-7 (Lane1), Hela (Lane2), and U937 (Lane3) cell lysate using PDPK1 antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of Hela cells using PDPK1 antibody

Western blot analysis of HEK293 using PDPK1 antibody

Quick Database Links

Gene Symbol

PDPK1

UniProt

O15530

NCBI Gene ID

5170