PDCD1 Antibody / PD-1 / PD1

• Catalog Number: orb640137
• Category: Antibodies
• Description: PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B-lymphocytes and myeloid cells. Additionally, Pdcd1 mRNA is expressed in developing B-lymphocytes during the pro-B-cell stage. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production co-stimulated by macrophages (but not by dendritic cells and B cells).
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Mouse
• Immunogen: PDCD1 transfected BHK cells were used as the immunogen for the PD1 antibody.
• UniProt ID: Q02242
• Tested applications: ELISA, FACS, IF, NeA
• Dilution range: ELISA (order BSA/azide-free format for coating),Neutralization Studies (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: RMP1-14
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Apoptotic, Cancer, Epigenetics, Immunology, Neuros
• Note: For research use only

SDS-PAGE analysis of purified, BSA-free PD1 antibody (clone RMP1-14) as confirmation of integrity and purity.